http://cfs.nrcan.gc.ca/authors/read/17292 Publications by M. Wettasinghe en-ca 2001-11-23 00:00:00 MST 2001-11-23 00:00:00 MST webmaster@nofc.cfs.nrcan.gc.ca http://cfs.nrcan.gc.ca/publications?id=18835 An ethanolic extract of borage meal was fractionated (fractions I-VI) on a Sephadex LH-20 column. All fractions tested positive for phenolics, but were negative for condensed tannins. Silica gel thin-layer chromatography (TLC) of fractions allowed the location of one and two strong antioxidative spots in fractions I and IV, respectively. Other fractions produced spots containing either weak antioxidative compounds or compounds with low concentrations. High perfomance liquid chromatography of the three major TLC spots in fractions I and IV showed the presence of one phenolic compound in each spot. Ultraviolet, proton nuclear magnetic resonance and proton-proton correlation of spectroscopies, as well as electron impact-mass spectrometry, allowed the identification of three phenolic acids, namely rosmarinic acid in fraction I, and syringic and sinapic acids in fraction IV. These three compounds contributed to 3.9% of the dry mass of the crude extract whereas their total contribution in the meal was 0.6% (w/w). Fri, 23 Nov 2001 http://cfs.nrcan.gc.ca/publications?id=18835