http://cfs.nrcan.gc.ca/authors/read/17983 Publications by Y. L'Homme en-ca 2002-08-08 00:00:00 MST 2002-08-08 00:00:00 MST webmaster@nofc.cfs.nrcan.gc.ca http://cfs.nrcan.gc.ca/publications?id=20479 We have used the conservation of reverse transcriptase and integrase domains among retroelements to PCR-amplify three well-known types of these mobile genetic elements. Reverse transcriptase sequences from Ty1-<em>copia</em> were identified in spruce in this way, as well as integrase sequences from the Ty3-<em>gypsy</em> group. Using these sequences as probes against a <em>Picea glauca</em> genomic bank, individual members from the LTR (long terminal direct repeat) groups were obtained. A partial Ty1-<em>copia</em>-type element named Spcl was isolated along with a Ty3-<em>gypsy</em>-type element named <em>Spdl</em>. Genomic Southern hybridizations revealed the complexity and high copy number of LTR retrotransposons in black and white spruce. Thu, 08 Aug 2002 http://cfs.nrcan.gc.ca/publications?id=20479