Canadian Forest Service Publications
Genetic transformaiton in conifers. 1995. Tsang, E.W.T.; Charest, P.J.; Sederoff, R.R. Pages 16-28 in P.J. Charest and L.C. Duchesne, compilers. Recent progress in forest biotechnology in Canada. Natural Resources Canada, Canadian Forest Service, Petawawa National Forestry Institute, Chalk River, Ontario, Information Report PI-X-120.
Available from: National Capital Region
Catalog ID: 21304
The most advanced breeding programs in conifers have so far gone only through a few generations of selection because of the long life cycle of these trees. This is one reason why the ability to transfer isolated genes into conifers and to produce transgenic trees could have a substantial impact on characteristics such as wood supply, wood fiber properties, and processing costs. Genetic engineering of conifers achieved success only 4 years ago and required the integration of tissue culture techniques such as somatic embryogenesis with a new gene transfer technique called microprojectile-mediated DNA delivery. (Agrobacterium-mediated DNA delivery proved ineffective). Other methods were also tested and yielded transient gene expressio but did not produce transgenic conifers. Microprojectile-mediated DNA delivery allowed several studies on gene expressio in various tissues of conifers, with particular emphasis on the evaluation of promoter regions from angiosperm genes. Also, reporter genes and selectable marker genes have been tested to improve gene transfer efficiency in conifers.