Canadian Forest Service Publications

Identification and Characterization of a Juvenile Hormone (JH) Response Region in the JH Esterase Gene from the Spruce Budworm, Choristoneura fumiferana. 2004. Kethidi, D.R.; Perera, S.C.; Zheng, S.; Feng, Q.L.; Krell, P.J.; Retnakaran, A.; Palli, S.R. Journal of Biological Chemistry 279: 19634-19642.

Year: 2004

Available from: Great Lakes Forestry Centre

Catalog ID: 26805

Language: English

CFS Availability: PDF (request by e-mail)

Abstract

Using a differential display of mRNA technique we discovered that the juvenile hormone (JH) esterase gene (Cfjhe) from Choristoneura fumiferana is directly induced by juvenile hormone I (JH I), and the JH I induction is suppressed by 20-hydroxyecdysone (20E). To study the mechanism of action of these two hormones in the regulation of expression of this gene, we cloned the 1270-bp promoter region of the Cfjhe gene and identified a 30-bp region that is located between –604 and –574 and is sufficient to support both JH I induction and 20E suppression. This 30-bp region contains two conserved hormone response element half-sites separated by a 4-nucleotide spacer similar to the direct repeat 4 element and is designated as a putative juvenile hormone response element (JHRE). In CF-203 cells, a luciferase reporter placed under the control of JHRE and a minimal promoter was induced by JH I in a dose- and time-dependent manner. Moreover, 20E suppressed this JH I-induced luciferase activity in a dose- and time-dependent manner. Nuclear proteins isolated from JH I-treated CF-203 cells bound to JHRE and the binding was competed by a 100-fold excess of the cold probe but not by 100-fold excess of double-stranded oligonucleotides of unrelated sequence. JH I induced/modified nuclear proteins prior to their binding to JHRE and 20E suppressed this JH I induction/modification. These results suggest that the 30-bp JHRE identified in the Cfjhe gene promoter is sufficient to support JH induction and 20E suppression of the Cfjhe gene.

Date modified: