Canadian Forest Service Publications
Genome of Sindbis Virus. 1972. Arif, B.M.; Faulkner, P. Journal of Virology 9: 102 - 109.
Available from: Great Lakes Forestry Centre
Catalog ID: 28919
P_labeled ribonucleic acid (RNA) from purified Sindbis viru s was examined for the presence of hidden breaks. Viral RNA was treated with acid at p H 2.9 or with forma ldehyde and was analyzed on sucrose gradients or by polyacryla mide gel electrophoresis. The sedimenlatio n pattern and mobility on polyacrylamide gels of the 425 RNA was unaffected by heating and quick cooling and was not a ltered by de naturing agents such as dimethyl sulfoxide and urea . No evide nce that Sindbis RNA is a pclyaggregate o f fragments was obtained It is concluded th at the genome consists of a continuous length of single-st randed polyn ucleot ide.