Canadian Forest Service Publications

Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis. 2008. Graham, R.I.; Morin, B.; Lapointe, R.; Nealis, V.G.; Lucarotti, C.J. Archives of Virology 153: 1759-1763.

Year: 2008

Available from: Atlantic Forestry Centre

Catalog ID: 28962

Language: English

CFS Availability: Order paper copy (free)

Abstract

A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis. The complete nucleotide sequences of genomic segments S2-S5 and S7-S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5’ (AGU-UU.....UUUGUGC) 3’ were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus with the family Reoviridae. Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana.