Canadian Forest Service Publications

Cloning and characterization of a protein kinase C gene from the spruce budworm, Choristoneura fumiferana. 2008. Quan, G.X.; Doucet, D.; Ladd, T.R.; Krell, P.J.; Arif, B.M. Insect Biochemistry and Molecular Biology 38: 984 - 992.

Year: 2008

Available from: Great Lakes Forestry Centre

Catalog ID: 29169

Language: English

CFS Availability: PDF (request by e-mail)

Abstract

Recent studies have implicated protein kinase C (PKC) in the control of 20-hydroxyecdysone (20E)- dependent gene expression during molting and metamorphosis in insects. To further understand the role of this kinase in 20E signal transduction, we cloned a homolog of mammalian PKC by RT-PCR and 50/30- RACE from adult of the moth Choristoneura fumiferana. The full-length cDNA of the C. fumiferana PKC (CfPKC1) is 2.3 kb with an open reading frame encoding a protein of 669 amino acids. The deduced amino acid sequence contains all the characteristic features of the classical protein kinase C subfamily. Northern and Western blot analysis showed that CfPKC1 was distributed ubiquitously in various tissues and at different developmental stages. Activation of CfPKC1 with the PKC activator phorbol 12-myristate 13- acetate (PMA) resulted in a rapid redistribution of the protein from the cytosol to the plasma membrane. Knock-down of the CfPKC1 gene by double-stranded RNA interference or treatment of the CF-203 cells with PKC-specific inhibitors reduces the expression of the 20E-responsive genes CHR3 and E75. This data suggests that CfPKC1 is involved in the 20E-response gene expression in C. fumiferana.