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Ectomycorrhizae Descriptions Database

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Collection Ecology and Identification

Species Information
Collection Name: GenBank Number: Collection Number: Herbarium Number:
Publication Data
Has it been published? : Citations :
Credits
Collected by: Described by: DNA Analysis by: Identified by: Re-identified by: Previous name: Photography by:
Date Information

Date collected
/ /
Date described
/ /
Date re-identified
/ /
Previous name
Collection Information
Stand age: Description of Location: Elevation: Ecological zone:
Latitude
° " '
Longitude
° " '
Silvicultural Practice
Second GrowthOld GrowthContainerBareroot Other:
Substrate
Decaying woodDecaying litterMineral SoilNursery mix Other:
Soil Horizon
LitterFragmented litterHumusA LayerB LayerC Layer
Frequency of Mycorrhizal Type
RareCommon Notes on Frequency :
Root Tips
Roughly, number examined:
Months of Observation

Months of Survey
JanFebMarAprMayJunJulAugSepOctNovDec
Months Ectomycorrhiza Observed
JanFebMarAprMayJunJulAugSepOctNovDec
Identification Procedure
Synthesis Pure culture Mycelium and strand comparison Physical Link Published Description DNA Association
General Notes
Notes on Collection, Ecology, and Identification :

Morphology of the Ectomycorrhizal System

Branching
Monopodial PinnateMonopodial PyramidalDichotomousTuberculateIrregularNot branchedCoralloid Other Branching: Notes on Branching of systems :
Tip Shape
StraightBeadedClub-shapedTortuousBent Other: Notes on tip shape :
Dimensions
Length of System (mm): ( - ) Median Length of Tips (mm): ( - ) Width of Tips (mm): ( - ) Width of Axes (mm): ( - ) Width of Tip Bases (mm): ( - ) Width of Tip Apices (mm): ( - )
Colour

Young Tips
Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Older Tips
Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Tip Apices
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour :
Texture
SmoothFinely grainyCoarsely grainyFeltyVelvetyWartyWoollyCottonyStringyShort spinyLong spiny Other: Notes on texture :
Lustre
MatteShinyReflective Other: Notes on lustre :
Visibility of Host Through Mantle
YesNo Notes on visibility of host through mantle :
Chemical Reactions of Whole Ectomycorrhizae

KOH
Presence of reaction of whole ectomycorrhizae to koh: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Melzer's
Presence of reaction of whole ectomycorrhizae to melzers: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Sulfovanillin
Presence of reaction of whole ectomycorrhizae to sulfovanillin: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Toluidine Blue
Presence of reaction of whole ectomycorrhizae to toluidine blue: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on Colour :
General Notes
Notes on Morphology of Ectomycorrhizal System :

Morphology of Mycelial Strands

Frequency
NoneRareCommon Notes on frequency :
Attachment
Restricted PointFlat angleDichotomous Other attachment: Notes on attachment of mycelial strands :
Colour
Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour :
Surface at 20x
SmoothHairy Other surface: Notes on the surface of mycelial strands :
Shape in Cross Section
FlatRound Other cross section shape: Notes on cross section shape :
Branching Frequency
NoneRareCommon Notes on Branching Frequency :
Dimensions
Diameter (µm): ( - )
General Notes
Notes on Morphology of Mycelial Strands :

Morphology of Emanating Hyphae

Frequency
NoneRareCommon Notes on frequency :
Shape
StraightCurvedTortuous Other shape: Notes on shape :
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on Colour :
General Notes
Notes on Morphology of Emanating Hyphae :

Anatomy of the General Features of the Mantle

Thickness
ThinMediumThick Notes on thickness of mantle :
Agerer's Type
ABCDEFGHIJKLMNOPQ Notes on agerer's type :
Hartig Net
Is Hartig net present? :
Chemical Reactions of Mantle Scrapings
KOH
Presence of reaction of mantle scrapings to koh: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Melzer's
Presence of reaction of mantle scrapings to melzers: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Sulfovanillin
Presence of reaction of mantle scrapings to sulfovanillin: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Toluidine Blue
Presence of reaction of mantle scrapings to toluidine blue: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on chemical reactions of mantle scrapings :
General Notes
Notes on Mantle Anatomy :

Anatomy of Specialized Cells

Frequency
NoneRareCommon Notes on frequency of specialized cells :
Type
LaticifersOleiferous cells Other type:
Dimensions
Length (µm): ( - ) Width (µm): ( - )
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on Colour :
Contents
ClearGranularOil-like bodies Notes on contents of specialized cells :
Chemical Reactions of Specialized Cells

KOH
Presence of reaction of specialized cells to koh: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Melzer's
Presence of reaction of specialized cells to melzers: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Sulfovanillin
Presence of reaction of specialized cells to sulfovanillin: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Toluidine Blue
Presence of reaction of specialized cells to toluidine blue: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on chemical reactions of specialized cells :
General Notes
Notes on specialized cells :

Anatomy of the Outer Layer of the Mantle

Type
Felt prosenchymaNet prosenchymaNet synenchymaInterlocking irregular synenchymaNon-interlocking irregular synenchymaRegular synenchyma Notes on type of mantle :
Matrix Materials
Are they present? : Description of Matrix Materials:
Dimensions of Cells or Hyphae
Cell length (µm): ( - ) Cell width (µm): ( - ) Wall Thickness (µm): ( - )
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour :
Shape
StraightCurvedTortuous Other shape: Notes on shape :
Ornamentation Frequency
NoneRareCommon Notes on ornamentation frequency :
Ornamentation Type
CrystalineVerrucoseGlobular Notes on ornamentation type :
Ornamentation Size
SmallMediumLarge Notes on ornamentation size :
Contents
ClearGranularOil-like bodies Notes on contents :
Septa Frequency
NoneRareCommon Notes on septa frequency :
Clamp Frequency
NoneRareCommon Notes on clamp frequency :
Hyphal Junction Frequency
NoneRareCommon Notes on hyphal junction frequency :
Hyphal Junction Angle
0°30°45°60°90°120° Notes on hyphal junction angle :
Enlarged Junction Frequency
NoneRareCommon Notes on enlarged junction frequency :
Anastomoses Frequency
NoneRareCommon Notes on anastomoses frequency :
Anastomoses Type
ContactH-shaped Notes on anastomoses type :
Clamped Anastomoses
Are the anastomoses clamped? :
General Notes
Notes on the anatomy of the outer layer of the mantle :

Anatomy of the Outer Middle Layer of the Mantle

Type
Felt prosenchymaNet prosenchymaNet synenchymaInterlocking irregular synenchymaNon-interlocking irregular synenchymaRegular synenchyma Notes on type of mantle :
Matrix Materials
Are they present? : Description of Matrix Materials:
Dimensions of Cells or Hyphae
Cell length (µm): ( - ) Cell width (µm): ( - ) Wall Thickness (µm): ( - )
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour :
Shape
StraightCurvedTortuous Other shape: Notes on shape :
Ornamentation Frequency
NoneRareCommon Notes on ornamentation frequency :
Ornamentation Type
CrystalineVerrucoseGlobular Notes on ornamentation type :
Ornamentation Size
SmallMediumLarge Notes on ornamentation size :
Contents
ClearGranularOil-like bodies Notes on contents :
Septa Frequency
NoneRareCommon Notes on septa frequency :
Clamp Frequency
NoneRareCommon Notes on clamp frequency :
Hyphal Junction Frequency
NoneRareCommon Notes on hyphal junction frequency :
Hyphal Junction Angle
0°30°45°60°90°120° Notes on hyphal junction angle :
Enlarged Junction Frequency
NoneRareCommon Notes on enlarged junction frequency :
Anastomoses Frequency
NoneRareCommon Notes on anastomoses frequency :
Anastomoses Type
ContactH-shaped Notes on anastomoses type :
Clamped Anastomoses
Are the anastomoses clamped? :
General Notes
Notes on the anatomy of the outer middle layer of the mantle :

Anatomy of the Inner Middle Layer of the Mantle

Type
Felt prosenchymaNet prosenchymaNet synenchymaInterlocking irregular synenchymaNon-interlocking irregular synenchymaRegular synenchyma Notes on type of mantle :
Matrix Materials
Are they present? : Description of Matrix Materials:
Dimensions of Cells or Hyphae
Cell length (µm): ( - ) Cell width (µm): ( - ) Wall Thickness (µm): ( - )
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour :
Shape
StraightCurvedTortuous Other shape: Notes on shape :
Ornamentation Frequency
NoneRareCommon Notes on ornamentation frequency :
Ornamentation Type
CrystalineVerrucoseGlobular Notes on ornamentation type :
Ornamentation Size
SmallMediumLarge Notes on ornamentation size :
Contents
ClearGranularOil-like bodies Notes on contents :
Septa Frequency
NoneRareCommon Notes on septa frequency :
Clamp Frequency
NoneRareCommon Notes on clamp frequency :
Hyphal Junction Frequency
NoneRareCommon Notes on hyphal junction frequency :
Hyphal Junction Angle
0°30°45°60°90°120° Notes on hyphal junction angle :
Enlarged Junction Frequency
NoneRareCommon Notes on enlarged junction frequency :
Anastomoses Frequency
NoneRareCommon Notes on anastomoses frequency :
Anastomoses Type
ContactH-shaped Notes on anastomoses type :
Clamped Anastomoses
Are the anastomoses clamped? :
General Notes
Notes on the anatomy of the inner middle layer of the mantle :

Anatomy of the Inner Layer of the Mantle

Type
Felt prosenchymaNet prosenchymaNet synenchymaInterlocking irregular synenchymaNon-interlocking irregular synenchymaRegular synenchyma Notes on type of mantle :
Matrix Materials
Are they present? : Description of Matrix Materials:
Dimensions of Cells or Hyphae
Cell length (µm): ( - ) Cell width (µm): ( - ) Wall Thickness (µm): ( - )
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour :
Shape
StraightCurvedTortuous Other shape: Notes on shape :
Ornamentation Frequency
NoneRareCommon Notes on ornamentation frequency :
Ornamentation Type
CrystalineVerrucoseGlobular Notes on ornamentation type :
Ornamentation Size
SmallMediumLarge Notes on ornamentation size :
Contents
ClearGranularOil-like bodies Notes on contents :
Septa Frequency
NoneRareCommon Notes on septa frequency :
Clamp Frequency
NoneRareCommon Notes on clamp frequency :
Hyphal Junction Frequency
NoneRareCommon Notes on hyphal junction frequency :
Hyphal Junction Angle
0°30°45°60°90°120° Notes on hyphal junction angle :
Enlarged Junction Frequency
NoneRareCommon Notes on enlarged junction frequency :
Anastomoses Frequency
NoneRareCommon Notes on anastomoses frequency :
Anastomoses Type
ContactH-shaped Notes on anastomoses type :
Clamped Anastomoses
Are the anastomoses clamped? :
General Notes
Notes on the anatomy of the inner layer of the mantle :

Anatomy of Mycelial Strands

Type
Loose-undifferentiated smooth-undifferentiated slightly-differentiated differentiated-random hyphae differentiated-central core highly differentiated Notes on type of mycelial strands :
Dimensions of Hyphae (most common type)
Cell Length (µm): ( - ) Cell Width (µm): ( - ) Cell Wall Thickness (µm): ( - )
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour of mycelial strand cells :
Shape
StraightCurvedTortuous Other: Notes on Shape :
Ornamentation Frequency
NoneRareCommon Notes on Ornamentation Frequency :
Ornamentation Type
CrystalineVerrucoseGlobular Notes on Ornamentation of Cells :
Ornamentation Size
SmallMediumLarge Notes on the Size of the Ornamentation of Cells :
Contents
ClearGranularOil-like bodies Notes on contents :
Septa Frequency
NoneRareCommon Notes on septa frequency :
Clamp Frequency
NoneRareCommon Notes on clamp frequency :
Hyphal Junction Frequency
NoneRareCommon Notes on hyphal junction frequency :
Hyphal Junction Angle
0°30°45°60°90°120° Notes on hyphal junction angle :
Enlarged Junction Frequency
NoneRareCommon Notes on enlarged junction frequency :
Anastomoses Frequency
NoneRareCommon Notes on anastomoses frequency :
Anastomoses Type
ContactH-shaped Notes on anastomoses type :
Clamped Anastomoses
Are the anastomoses clamped? :
Chemical Reactions of Mycelial Strands

KOH
Presence of reaction of mycelial strands to koh: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Melzer's
Presence of reaction of mycelial strands to melzers: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Sulfovanillin
Presence of reaction of mycelial strands to sulfovanillin: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Toluidine Blue
Presence of reaction of mycelial strands to toluidine blue: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on chemical reactions of mycelial strands :
General Notes
Notes on the anatomy of Mycelial Strands in Plan View :

Anatomy of Emanating Hyphae

Frequency
NoneRareCommon Notes on frequency :
Dimensions
Cell Length (µm): ( - ) Cell Width (µm): ( - ) Cell Wall Thickness (µm): ( - )
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour of emanating hyphae cells :
Shape
StraightCurvedTortuous Other: Notes on Shape :
Ornamentation Frequency
NoneRareCommon Notes on Ornamentation Frequency :
Ornamentation Type
CrystalineVerrucoseGlobular Notes on Ornamentation of Cells :
Ornamentation Size
SmallMediumLarge Notes on the Size of the Ornamentation of Cells :
Contents
ClearGranularOil-like bodies Notes on contents :
Septa Frequency
NoneRareCommon Notes on septa frequency :
Clamp Frequency
NoneRareCommon Notes on clamp frequency :
Hyphal Junction Frequency
NoneRareCommon Notes on hyphal junction frequency :
Hyphal Junction Angle
0°30°45°60°90°120° Notes on hyphal junction angle :
Enlarged Junction Frequency
NoneRareCommon Notes on enlarged junction frequency :
Anastomoses Frequency
NoneRareCommon Notes on anastomoses frequency :
Anastomoses Type
ContactH-shaped Notes on anastomoses type :
Clamped Anastomoses
Are the anastomoses clamped? :
General Notes
Notes on the anatomy of Emanating Hyphae :

Anatomy of Cystidia

Frequency
NoneRareCommon Notes on frequency :
Type
Bristle-like, awl-shapedBottle-shaped with straight neckFusiformBristle-like with apical lobesBottle shaped with bent neckGlobularCapitateThin walled, slightly acuminateFlask-shaped with apical knobAcanthocystidiumRamifiedLateral with tapering, capitate branchBottle-shaped with finger-like outgrowthsSickle-shaped, with thick walls Other: Notes on type of cystidia :
Dimensions
Length (µm): ( - ) Apical Width (µm): ( - ) Medial Width (µm): ( - ) Basal Width (µm): ( - ) Wall Thicknes (µm): ( - )
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour of cystidia :
Ornamentation Frequency
NoneRareCommon Notes on Ornamentation Frequency :
Ornamentation Type
CrystalineVerrucoseGlobular Notes on Ornamentation of Cystidia :
Ornamentation Size
SmallMediumLarge Notes on the Size of the Ornamentation of Cystidia :
Contents
ClearGranularOil-like bodies Notes on contents :
Septa Frequency
NoneRareCommon Notes on septa frequency :
Frequency of Clamped Septa
NoneRareCommon Notes on clamped septa frequency :
Location of Clamps
Location of Clamps Notes on clamp location :
Chemical Reactions of Cystidia

KOH
Presence of reaction of cystidia to koh: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Melzer's
Presence of reaction of cystidia to melzers: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Sulfovanillin
Presence of reaction of cystidia to sulfovanillin: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Toluidine Blue
Presence of reaction of cystidia to toluidine blue: Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on chemical reactions of cystidia :
General Notes
Notes on cystidia :

Anatomy of Sclerotia

Frequency
NoneRareCommon Notes on frequency :
Dimensions
Diameter: (µm): ( - )
Colour
Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour of sclerotia :
General
Shape: Texture: Notes on sclerotia and microsclerotia :

Anatomy of Chlamydospores

Frequency
NoneRareCommon Notes on frequency :
Shape
GloboseSubgloboseBroadly ellipsoidalEllipsoidalOvalFusiformFiliform AceroseCylindricalReniformAllantoidLunateFalcateOvoidPyriform ClavateDoliform Other shape: Notes :
Dimensions
Length: (µm): ( - ) Width: (µm): ( - )
Colour
Intensity: Dark Medium Light Hyaline Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow Notes on colour :
Surface
SmoothVerrucoseReticulateStriateEchinulate (spiny)WartedTuberculate Other shape: Notes on surface :
Hyphal Attachment
TerminalIntercalaryDetached Notes on attachment :
Wall Thickness
Wall Thickness: (µm): ( - )
General Notes
Notes on Chlamydospores :

Autofluorescence of Whole Tips

Autofluorescence at 254nm
Present? : Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
Autofluorescence at 366nm
Present? : Intensity: Dark Medium Light Colours: Black Blue Brown Green Grey Orange Pink Purple Red White Yellow
General Notes
Notes on autofluorescence :

DNA Description

CDE Standard RFLP
Region targeted: ITS Primers set: ITS1/NL6Bmun
Enzyme: alui
Fragment Lengths: , , , , , , ,
Enzyme: hinfi
Fragment Lengths: , , , , , , ,
Enzyme: rsai
Fragment Lengths: , , , , , , ,
Other RFLP
Region Targeted:: First Primer:: Second Primer::
Enzyme 1
Enzyme: Fragment Lengths: , , , , , , ,

Enzyme 2
Enzyme: Fragment Lengths: , , , , , , ,
Enzyme 3
Enzyme: Fragment Lengths: , , , , , , ,
Enzyme 4
Enzyme: Fragment Lengths: , , , , , , ,
Sequence availability
Is a sequence available? : Region Sequenced:
GenBank
Additional GenBank numbers :
General Notes
Notes on DNA Description :

Additional Characters

Hartig Net in Cross Section
Common Coarse Ramified haustoria Globular haustoria Beaded Other: Notes on hartig net :
Other
Other Characters :
Distinguishing Features
:

External References

References
:

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