Canadian Forest Service Publications
Root induction in hybrid poplar by Agrobacterium genetic transformation. 1992. Charest, P.J.; Stewart, D.; Budicky, P.L. Canadian Journal of Forest Research 22(12): 1832-1837.
Issued by: National Capital Region
Catalog ID: 10741
Availability: PDF (request by e-mail)
Three Agrobacterium rhizogenes strains (A4, 8196, and ATC 39207) and two Agrobacterium tumefaciens mutant strains (A208 (pTiT37.14a/a) and Cf8 (pGV3297)) were evaluated for their genetic transformation frequency in vitro on cuttings of Populus deltoides x nigra line DN 106 and Populus nigra x maximowiczii lines NM1 and NM6. The aim of this work was to increase biomass production by genetically modifying hybrid poplar root systems. Inoculation of the cut end of in vitro grown stems with the A. rhizogenes strain ATC 39207 gave the highest frequency of typical hairy root formation with line DN106. the other combinations of Agrobacterium strains and poplar lines yielded very low frequencies of hairy root induction or induction of tumours instead of hairy roots. Similar attempts with greenhouse-grown plants gave negative results. The modified root system resulting from the in vitro inoculation of line DN106 cuttings yielded an increase in root and aerial biomass production after 175 days of growth in vitro. Preliminary data confirm this observation in the greenhouse. Genetic transformation of the tissues was proven by phenotype (hairy root or tumour), hormone autotrophy, and opine production in the resulting tissues. Because of the host range restriction and inefficiency of this approach, it is not practical at this time for use in the transformation of hybrid poplar.