Canadian Forest Service Publications
Microprojectile-mediated DNA delivery in haploid and diploid embryogenic cells of Larix spp. 1993. Duchesne, L.C.; Lelu, M.-A.; von Aderkas, P.; Charest, P.J. Canadian Journal of Forest Research 23: 312-316.
Issued by: National Capital Region
Catalog ID: 10780
Availability: PDF (request by e-mail)
A particle bombardment protocol using the Dupont Biolistic TM particle delivery system and the -glucuronidase reporter gene was developed for embryogenic cell lines of Larix. Comparison of four sizes of tungsten microprojectiles showed the highest transient gene expression with 1.11 m diameter particles. The highest -glucuronidase transient gene activity was in cells bombarded 5 and 6 days after subculturing to fresh media. Comparison of 22 different cell lines of haploid and diploid Larix decidua, Larix leptolepis, Larix xleptoeuropae, and Larix xeurolepis showed variation among the cell lines in -glucuronidase gene activity, embryogenesis, and cell growth. In diploid lines, the levels of transient gene expression were higher with youngest lines; however, this trend was not distinguishable in haploid lines.