Canadian Forest Service Publications

An improved method for somatic plantlet production in hybrid larch (Larix xleptoeuropaea): Part 2. Control of germination and plant development. 1994. Lelu, M.-A.; Bastien, C.; Klimaszewska, K.; Charest, P.J. Plant Cell Tissue and Organ Culture 36: 117-127.

Year: 1994

Issued by: National Capital Region

Catalog ID: 10814

Language: English

Availability: PDF (request by e-mail)

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Germination and plantlet development in somatic embryos of Larix xleptoeuropaea were affected by the duration of the maturation treatment and the concentrations of sucrose and abscisic acid in the maturation media. Extension of the maturation period from 3 weeks to 4 weeks resulted in a significant decrease in germination and plantlet development frequencies. There was no significant effect of abscisic acid concentration on either the number of somatic embryos germinated or the number of plantlets obtained, but it affected the rapidity of the epicotyl development. Sucrose of 0.2 M, applied during maturation, was significantly more beneficial in attaining high germination rated that at 0.1 M. High germination rated (92 and 93%) and plantlet development rated (74 and 80%) were achieved when somatic embryos were matured for a 3-week period on media with either 40 or 60 M abscisic acid, respectively, and 0.2 M sucrose prior to transfer to the growth regulator-free germination medium. Two acclimatization methods were applied: the first required 10 to 12 weeks and ensured 97% plantlet survival under greenhouse conditions; the second required 2-3 weeks and ensured 86% plantlet survival. This represents the first detailed study of the effects of maturation regimes on the recovery of somatic embryo-derived plants of Larix.