Canadian Forest Service Publications

An improved method for somatic plantlet production in hybrid larch (Larix xleptoeuropaea): Part 1. Somatic embryo maturation. 1994. Lelu, M.-A.; Bastien, C.; Klimaszewska, K.; Ward, C.; Charest, P.J. Plant Cell Tissue and Organ Culture 36: 107-115.

Year: 1994

Issued by: National Capital Region

Catalog ID: 10815

Language: English

Availability: Not available through the CFS (click for more information).

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Somatic embryogenesis was induced from full-sib immature zygotic embryos of hybrid larch (Larix xleptoeuropaea) that were collected at three different dates. Analysis of variance showed interaction between the collection date and the induction medium. The highest response (55%) was observed from embryos that were at the precotyledonatry stage. Twelve media containing various concentrations of abscisic acid and sucrose were used to promote the development of ‘high quality' mature somatic embryos that would undergo a period of developmental arrest. Only media supplemented with abscisic acid (20, 40, and 60 uM), indolebutyric acid (1 uM), and 0.1 or 0.2 M sucrose supported such a development. The number of mature somatic embryos produced per gram fresh weight of embryonal mass was significantly affected by the three factors tested: embryogenic line, sucrose concentration, and abscisic acid concentration. Moreover, strong interaction effects amond these factors existed, complicating the formulation of a universal maturation medium that would be optimal for all embryogenic lines.