Canadian Forest Service Publications
Accumulation of mansones E and F in elm callus cultures inoculated with Ophiostoma ulmi. 1994. Duchesne, L.C.; Jeng, R.S.; Hubbes, M.; Sticklen, M.B. Canadian Journal of Plant Pathology 16: 118-121.
Issued by: National Capital Region
Catalog ID: 10854
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The accumulation of mansonone E and F in relation to susceptibility of elm to Dutch elm disease, caused by Ophiostoma ulmi, was investigated in callus clutures of Ulmus americana (susceptible), U. pumila (resistant), U. carpinifolia (intermediate resistant) and U. x ‘Pioneer' (resistant). Callus cultures of each elm species were maintained on modified B5 or modified Murashige and Skoog media in petri dishes, inoculated with either an aggressive or a nonaggressive isolate of O. ulmi, and analyzed for mansonone content 12, 24, and 48 h later. Mansonone accumulation in elm callus cultures depended on the medium, the host species, the fungal isolate, and time. Only mansonones E and F were observed, and in measurable concentrations only in U. americana and U. pumila grown on modified B5 medium. Mansonones were detected soonest in callus cultures inoculated with the nonagressive isolate (after 12 h in U. pumila and after 24 h in U. americana). At 48 h, mansonone concentrations reached their highest level, were higher in U. pumila (322-400 g/g) than in U. americana (29-31 g/g), and were not significantly affected by the isolate of O. ulmi. There was no relation between mansonone accumulation in callus cultures and resistance of elm to Dutch elm disease.