Canadian Forest Service Publications

The use of polyacrylamide gel electrophoresis for rapid differentiation of Gremmeniella abietina isolates. 1984. Benhamou, N.; Ouellette, G.B.; Asselin, A.; Maicas, E. Pages 68-76 in P.D. Manion, editor. Scleroderris Canker of Conifers: Proceedings of an international symposium on scleroderris canker of conifers, June 21-24, 1983, Syracuse, New York, USA. Kluwer Publishing, Boston, Massachusetts, USA.

Year: 1984

Issued by: Laurentian Forestry Centre

Catalog ID: 14282

Language: English

Availability: PDF (download)

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Soluble proteins from crude mycelial extracts of Gremmeniella abietina isolates from Europe, North America and Japan were compared using non gradient and gradient polyacrylamide gel-slab electrophoresis. With Coomassie blue, a total of 18 bands were recognized when results from all isolates were grouped (standard type). With reference to this standard type, isolates could be classified into six groups: 1) Isolates from Europe, New York State (US7-US11), and some from red pine from Quebec, are characterized by the presence of band 12 and the absence of bands 4 and 10. 2) Isolates from Ontario (C1) and others from pine from Quebec, show the presence of band 4 and the absence of bands 10 and 12. 3) Isolates from Japan lack band 12. 4) Isolates from New Brunswick and some from Quebec (MER) show the greatest number of bands and combining both European and American characteristics. 5) Isolates from spruce from Quebec are relatively similar to the isolates from the second group but distinguishable by at least three additional bands. 6) Isolates from balsam fir from Quebec are characterized by the lack of several bands and especially band 13. The importance and the interest of these two tests as complementary or even as a possible substitute to serology is discussed.