Canadian Forest Service Publications

Immunocytochemical localization of antigen-binding sites in the cell surface of two ascomycetous fungi using antibodies produced against fimbriae from Ustilago violacea and Rhodoturola rubra. 1986. Benhamou, N.; Ouellette, G.B.; Gardiner, R.; Day, A.W. Can. J. Microbiol. 32: 871-883.

Year: 1986

Issued by: Laurentian Forestry Centre

Catalog ID: 14293

Language: English

Availability: PDF (request by e-mail)

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Cross-reactivity between antisera produced against fimbriae of either Ustilago violacea (Pers.) Fuck. (AU) or of Rhodotorula rubra (Demme) Lodder (AR) and cell surface proteins of two ascomycete fungi, Ascocalyx abietina (Lagerberg.) Schlaepfer-Bernhard and Ophiostoma ulmi (Buism.) Nannf., was revealed by means of dot-immunobinding and immunocytochemical methods. Following treatment with antiserum AR, the walls, septa, and plasma membrane of A. abietina and O. ulmi cells (the latter either in culture or grown in elm wood section) were appreciably labeled by gold particles, but the labeling intensity was always found to be greater over the plasma membrane. The fibrillar sheath surrounding cells of A. abietina reacted with antiserum AU while all other structures did not. No significant labeling with this antiserum occurred over cells of O. ulmi indicating that they either lacked these antigens or that these were more easily removed during the fixation process. Possible explanations for the differences obtained with antisera AR and AU are discussed along with an overview of the potential use of these antisera in conjunction with the protein A - gold method in studies of host - parasite interrelationships.