Canadian Forest Service Publications

Investigations on new means of identifying races of Ascocalyx abietina. 1988. Ouellette, G.B.; Benhamou, N.; Lecours, N.; Lachance, D. Pages 73-80 in E. Donaubauer and B.R. Stephan, compilers. Recent Research on Scleroderris Canker of Conifers. IUFRO Working Party S2.06-02. Proceedings of Meeting, September 2-6, 1986, Salsburg, Austria and Ljubjana, Yugoslavia. Wien: Forstliche Bundesversuchsanstalt, Vienna, Austria.

Year: 1988

Issued by: Laurentian Forestry Centre

Catalog ID: 14746

Language: English

Availability: PDF (download)

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Abstract

Results of testing isolates of Ascocalyx abietina (Lagerb.) Schlaepfard-Bernhard with electrophoresis (PAGE) on a large scale are presented. Using US11 as reference, characteristic bands of the 155 isolates tested were obtained that permitted separating these into two distinct groups. When methods of identification by serology and electrophoresis were compared, a 96% concordance was obtained between 129 isolates tested by the first and then by the second method, and 100% concordance in a reciprocal test using 26 other isolates. Variations of protein profiles in the group classified as North American call for further investigations. The value of PAGE for routine testing of isolates to differentiate the European race is discussed. A report is also given of the successful use of some of the specific protein bands eluted from the gels, to produce monoclonal antibodies.