Canadian Forest Service Publications
Pollen viability testing storage and related physiology. 1974. Binder, W.D.; Mitchell, G.M.; Ballantyne, D.J. Government of Canada. Department of the Environment. Canadian Forest Service, Pacific Forest Research Centre, Victoria, BC. Information Report BC-X-105. 37 p.
Issued by: Pacific Forestry Centre
Catalog ID: 1490
Series: Information Report (PFC - Victoria)
Availability: PDF (download)
This review of literature is concerned with the physiology and techniques involved in viability testing and storage of pollen, especially conifer pollen. Techniques involving pollen germination and staining are considered. Methods of pollen storage outlined include refrigeration, controlled humidity, freeze drying and vacuum storage. Related physiological topics discussed involve the identification and quantitative determination of carbohydrates, proteins, amino acids, enzymes and isozymes, as well as the measurement of respiration rates of pollen. Suggestions involving the use of physiological parameters to determine conifer pollen viability have been made by the authors. The authors have also considered improvements in current methods of conifer pollen storage which might be investigated.