Canadian Forest Service Publications
Pectic enzyme patterns as a taxonomic tool for the characterization of Gremmeniella spp. isolates. 1994. Lecours, N.; Toti, L.; Sieber, T.; Petrini, O. Can. J. Bot. 72: 891-896.
Available from: Laurentian Forestry Centre
Catalog ID: 16521
CFS Availability: PDF (request by e-mail)
Isolates of different species of the conifer pathogen Gremmeniella, including some cultures from western Canada and some endophytic isolates, have been characterized by pectic enzyme patterns. Pectinase isozyme patterns are in good agreement with results of electrophoresis of mycelial water-soluble proteins. The North American and European races of G. abietina var. abietina can be distinguished on the basis of a cathodal polygalacturonase band present in all isolates of the former but absent in the latter. Another band, present in all isolates, shows polymorphisms related to the host from which the isolates are derived, thus making it possible to group them on a pathogen race - host genus basis. Sixteen isolates originating from western Canada that were previously found to belong to the North American race with a water-soluble protein assay were also found to belong to that race following the analysis of pectic enzymes. It was not possible using our assay to distinguish between isolates of Brunchorstia pinea var. cembrae and others derived from other Pinus spp. Among the endophytic isolates, the Pinus cembra endophytes show the same polygalacturonase profile as those from diseased pines.
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