Canadian Forest Service Publications

Isozyme and RAPD polymorphisms in Heterobasidion annosum in Italy. 1998. Goggioli, V.; Capretti, P.; Hamelin, R.C.; Vendramin, G.G. European Journal of Forest Pathology 28: 63-74.

Year: 1998

Issued by: Laurentian Forestry Centre

Catalog ID: 16770

Language: English

Availability: PDF (request by e-mail)

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Abstract

Isozyme and random amplified polymorphic DNA (RAPD) polymorphisms were used to study variability in a group of 41 isolates from the Italian population of Heterobasidion annosum. The isolates belonged to the intersterility groups P and S, and particularly to the group that is most widely distributed in Italy, group F. Isozyme analysis was effective in identifying the three intersterility groups and revealed a high degree of genetic divergence within the P group isolates; the mannose phosphate isomerase (MPI-2) locus was diagnostic in the attribution of isolates to the more correlated F and S groups. RAPDs were detected following amplification by the polymerase chain reaction (PCR). 74 RAPD fragments, obtained through amplifications with eight primers, were scored. Isolates from the 3 intersterility groups were clearly divergent based on analysis of RAPD markers. However, a similarity index calculated for the isolates within the F population indicated a high uniformity of the isolates collected throughout the Italian peninsula.