Canadian Forest Service Publications
Isolation and characterization of a cDNA clone encoding a putative white spruce glycine-rich RNA binding protein. 1999. Richard, S.; Drevet, C.; Jouanin, L.; Séguin, A. Gene 240(2): 379-388.
Available from: Laurentian Forestry Centre
Catalog ID: 16874
CFS Availability: PDF (request by e-mail)
A presumably full-length cDNA encoding a putative glycine-rich RNA binding protein was isolated from a λZAP cDNA library prepared from mRNAs extracted from needles of 2 year old white spruce seedlings, which had been either wounded or jasmonate-treated. The cDNA, designated PgRNP (Picea glauca RNP protein), presents a 468 bp open reading frame encoding a 155 amino acid protein. This polypeptide possesses an RNA binding domain (RNP-CS) and a glycine-rich domain. Comparative alignment reveals extensive homologies to glycine-rich RNA binding proteins containing an RNP-CS found in other angiosperm species. Genomic hybridization experiments suggest that the PgRNP gene is part of a small multigene family with at least four members. RNA blot analysis revealed that the PgRNP transcript is expressed in all tissues from non-stressed plants. Constitutive mRNA level was found in needle tissue from control as well as methyl-jasmonate treated plants. Wounding had no clear induction effect. Jasmonic acid treatment and systemic wound response had a positive effect on transcript accumulation. Transcript accumulation was slightly induced by cold in needles, and repressed by drought stress in both needle and root tissues of 2 year old plants. Finally, the level of PgRNP accumulation was induced by wounding and repressed in 2 week old dark-grown seedlings upon jasmonate treatments.
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