Canadian Forest Service Publications
Isozyme assessment of the genetic stability of micropropagated hybrid larch (Larix x eurolepis Henry). 1995. Richard, S.; Gauthier, S.; Laliberté, S. Can. J. For. Res. 25: 1103-1112.
Issued by: Laurentian Forestry Centre
Catalog ID: 16934
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The search for the occurrence of somaclonal variation of in vitro shoots and acclimatized plants of a hybrid larch (Larix x eurolepis Henry) clone was performed by the analysis of eight isozyme systems. Cultures were established from short shoot buds of mature material. The effects of growth regulators in the media, subculture intervals, and periods in culture were analyzed for in vitro shoots. Variability was found in in vitro shoots but appeared to be related to a physiological response to culture conditions. Once acclimatized, most tissue-cultured plants expressed the same enzymatic patterns as those of control plants (stecklings and the ortet). The variations observed for some acclimatized plants were also observed in control plants and were not related to ontogenic stage. Results from the isoenzymatic systems studied showed that hybrid larch plants regenerated from tissue culture were not significantly different from stecklings and the ortet.