Canadian Forest Service Publications
Ultrastructural localization of chitin in the five spore stages of the blister rust fungus, Cronartium ribicola. 2000. Ekramoddoullah, A.K.M.; Jensen, G.D.; Manning, L.E. Mycological Research 104(11): 1384-1388.
Issued by: Pacific Forestry Centre
Catalog ID: 18020
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Ultrathin sections of five spore stages of C. ribicola were incubated with wheat germ agglutinin (WGA), which binds to chitin, followed by an ovomucoid-gold complex, which binds to WGA and examined by TEM. Labeling experiments showed the presence of chitin in all five stages of the fungus, although the distribution of chitin varied among spore stages. Competitive inhibition studies with triacetyl chitotriose showed that WGA bound to cell walls of basidiospores much more strongly than other spore types, requiring a 10 fold increase in concentration over that required for the other spore types to achieve complete inhibition.