Canadian Forest Service Publications

In vitro germination and development of western hemlock dwarf mistletoe. 2001. Deeks, S.J.; Shamoun, S.F.; Punja, Z.K. Plant Cell, Tissue and Organ Culture 66(2): 97-105.

Year: 2001

Issued by: Pacific Forestry Centre

Catalog ID: 18372

Language: English

Availability: Not available through the CFS (click for more information).

Available from the Journal's Web site.
DOI: 10.1023/A:1010663108233

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A procedure for in vitro culture of the parasitic flowering plant western hemlock dwarf mistletoe, Arceuthobium tsugense (Rosend.) G.N. Jones subsp. tsugense, is described. A factorial experiment evaluated the effects of media (Harvey‘s medium (HM) and modified White‘s medium (WM)), temperatures (15 °C and 20 °C), presence or absence of light, and plant growth regulators (the auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the cytokinin 6-benzylaminopurine (BAP) at varying concentrations (0.001 mg l^-1 to 1 mg l^-1)). Seed explants germinated in less than one week in culture and produced radicles. Optimal conditions for radicle elongation were WM at 20 °C in the presence of light and without plant growth regulators. Some of the radicles split at the tip to yield callus while others swelled to become spherical holdfasts. Holdfasts were also produced at the tips of radicles, and callus arose from split holdfasts. Factors that promoted holdfast production were Harvey‘s medium, light, and 2,4-D at 1 mg l^-1. Callus development from split radicles and split holdfasts was optimal on WM with 0.5 mg l^-1 2,4-D and 1 mg l^-1 BAP at 20 °C in the dark.