Canadian Forest Service Publications

Armillaria sinapina in herbaceous plant material from a peatland in Alberta, Canada. 2001. Thormann, M.N.; Myrholm, C.L.; Mallett, K.I. Canadian Journal of Botany 79(5): 643-647.

Year: 2001

Issued by: Northern Forestry Centre

Catalog ID: 18548

Language: English

Availability: Order paper copy (free), PDF (request by e-mail)

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Armillaria sinapina Bérubé & Dessureault mycelium was isolated once from living Carex aquatilis Wahlenb. rhizomes, twice from decomposing C. aquatilis leaves, and three times from decomposing Salix planifolia Pursh leaves in a southern boreal sedge-dominated fen in Alberta, Canada. Restriction fragment length polymorphism (RFLP) analyses of the IGS-1 region with the AluI restriction enzyme were used to identify the isolates, because conspicuous, epigeous basidiomes could not be found in or near the fen. During these analyses, two previously unpublished fragment patterns for A. sinapina were found, consisting of 399–240–135 base pairs (bp) and 399–240–183–135 bp. Interspecific somatic incompatibility and interfertility tests confirmed the identifications obtained from the RFLP analyses. This is the first report of an annulate species of Armillaria being actively involved in the colonization and decomposition of herbaceous plant material in an ecosystem having only an organic soil horizon. Surveys for species of Armillaria should be expanded to include peatlands and herbaceous plant materials, because they may serve as vast potential biomass and genetic diversity reservoirs for this genus.