Canadian Forest Service Publications
Phenolic acids in defatted seeds of borage (Borago officinalis L.) 2001. Wettasinghe, M.; Shahidi, F.; Amarowicz, R.; Abou-Zaid, M.M. Food Chemistry 75: 49-56.
Available from: Great Lakes Forestry Centre
Catalog ID: 18835
CFS Availability: PDF (request by e-mail)
An ethanolic extract of borage meal was fractionated (fractions I-VI) on a Sephadex LH-20 column. All fractions tested positive for phenolics, but were negative for condensed tannins. Silica gel thin-layer chromatography (TLC) of fractions allowed the location of one and two strong antioxidative spots in fractions I and IV, respectively. Other fractions produced spots containing either weak antioxidative compounds or compounds with low concentrations. High perfomance liquid chromatography of the three major TLC spots in fractions I and IV showed the presence of one phenolic compound in each spot. Ultraviolet, proton nuclear magnetic resonance and proton-proton correlation of spectroscopies, as well as electron impact-mass spectrometry, allowed the identification of three phenolic acids, namely rosmarinic acid in fraction I, and syringic and sinapic acids in fraction IV. These three compounds contributed to 3.9% of the dry mass of the crude extract whereas their total contribution in the meal was 0.6% (w/w).
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