Canadian Forest Service Publications
Establishment and characterization of Rubus tissue culture systems for in vitro bioassays against phytotoxins from Rubus fungal pathogens. 2002. Hollman, P.J.; Lohbrunner, G.K.; Shamoun, S.F.; Lee, S.P. Plant Cell Tissue And Organ Culture 68(1): 43-48.
Issued by: Pacific Forestry Centre
Catalog ID: 18877
Availability: Order paper copy (free)
The Rubus species R. parviflorus, R. spectabilis and R. strigosus interfere with conifer seedling establishment on forest regeneration sites in Canada and the United States. As a first step towards microbial metabolite-based control, callus and cell suspension cultures of the Rubus species were developed as a bioassay system to detect phytotoxic compounds that may have relevance in a vegetation control context. Rapidly growing friable callus and suspension cultures were obtained from leaf disks of the three weedy Rubus species using similar culture media conditions (modified Murashige and Skoog) but required different plant growth regulators (R. parviflorus, 4.5 microM 2,4-D; R. spectabilis, 26.9 microM NAA/0.5 microM zeatin; and R. strigosus, 12.4 microM picloram). Cell growth and health attributes including callus circumference, degree of browning and suspension culture cell viability as measured by the TTC vital stain assay were developed and were rapid and convenient to use. We have established Rubus tissue culture systems that will make it possible for large scale screening of phytotoxic metabolites.