Canadian Forest Service Publications

Different classes of retrotransposons in coniferous spruce species. 2000. L'Homme, Y.; Séguin, A.; Tremblay, F.M. Genome 43: 1084-1089.

Year: 2000

Issued by: Laurentian Forestry Centre

Catalog ID: 20479

Language: English

Availability: PDF (request by e-mail)

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We have used the conservation of reverse transcriptase and integrase domains among retroelements to PCR-amplify three well-known types of these mobile genetic elements. Reverse transcriptase sequences from Ty1-copia were identified in spruce in this way, as well as integrase sequences from the Ty3-gypsy group. Using these sequences as probes against a Picea glauca genomic bank, individual members from the LTR (long terminal direct repeat) groups were obtained. A partial Ty1-copia-type element named Spcl was isolated along with a Ty3-gypsy-type element named Spdl. Genomic Southern hybridizations revealed the complexity and high copy number of LTR retrotransposons in black and white spruce.