Canadian Forest Service Publications

Rapid microsatellite analysis using discontinuous polyacrylamide gel electrophoresis. 2002. White, E.E.; Sahota, R.; Edes, S. Genome 45: 1107-1109.

Year: 2002

Issued by: Pacific Forestry Centre

Catalog ID: 21234

Language: English

Availability: Order paper copy (free), PDF (request by e-mail)

Available from the Journal's Web site.
DOI: 10.1139/g02-084

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Abstract

A method for screening large numbers of samples for microsatellites using discontinuous, non-denaturing polyacrylamide gels and rapid fluorescent gel staining is described. Disc electrophoresis on slab gels provides high-resolution of PCR products. It is useful for collecting population data once microsatellite loci have been characterized.