Canadian Forest Service Publications

A MADS-Box gene specifically expressed in the reproductive tissues of red pine (Pinus resinosa) is a homologue to floral homeotic genes with C-function in angiosperms. 2003. Liu, J.-J.; Ekramoddoullah, A.K.M.; Podila, G.K. Physiology and Molecular Biology of Plants 9(2): 197-206.

Year: 2003

Available from: Pacific Forestry Centre

Catalog ID: 22766

Language: English

CFS Availability: Order paper copy (free)

Abstract

One cDNA clone, PMADS2, putatively encoding a MADS box protein (transcription factor), was isolated and characterized from immature female cones of gymnosperm red pine (Pinus resinosa). PMADS2 encoded a single polypeptide of 222 amino acids, which shared high similarity ranging from 57% to 99% with members of AG/PLE subfamily from species of angiosperms as well as other gymnosperms. Southern blot analysis showed that PMADS2 was a single copy gene in the genome of red pine. Northern blot analysis demonstrated that PMADS2 was expressed only in immature male and female cones, and the transcript was not detectable in vegetative tissues of needles, stems, roots, and shoots. The analyses of sequence similarity and expression pattern suggest that PMADS2 may be a red pine homologue to members of AG/PLE gene subfamily.

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