Canadian Forest Service Publications
Prevalence of baculoviruses in spruce budworm (Lepidoptera: Tortricidae) populations in New Brunswick. 2004. Lucarotti, C.J.; Eveleigh, E.S.; Royama, T.; Morin, B.; McCarthy, P.C.; Ebling, P.M.; Kaupp, W.J.; Guertin, C.; Arella, M. The Canadian Entomologist 136: 255-264.
Issued by: Great Lakes Forestry Centre
Catalog ID: 24256
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Outbreak and declining populations of spruce budworm (Choristoneura fumiferana (Clem.)) were sampled extensively at three locations in New Brunswick, Canada, between 1982 and 1992 and were examined for the prevalence of granulosis and nuclear polyhedrosis viruses (Baculoviridae). Larvae, pupae, and adults were collected using a variety of methods. Spruce budworm nuclear polyhedrosis virus (CfMNPV) genomic DNA probes and wet-mount light microscopy were used to determine CfMNPV prevalence in 50 274 juvenile spruce budworms. Spruce budworm granulosis virus (ChfuGV) genomic DNA probes were used to determine the prevalence of ChfuGV in 25 703 of these same samples. The prevalence of both viruses was low, with ChfuGV and CfMNPV not found in more than 15% and 2%, respectively, of samples in any collection in a given year. Prevalence of ChfuGV was greatest in mid- to late June in sixth-instar larvae. Each virus was detected in only two of 2177 female moths and in none of the 420 male moths examined. In the entire collection, cytoplasmic polyhedrosis virus (Reoviridae) was detected in only two budworm larve and entomopoxvirus (Poxviridae) was not detected in any.