Canadian Forest Service Publications

Characterization of a MYBR2R3 gene from black spruce (Picea mariana) that shares functional conservation with maize C1. 2003. Xue, B.; Charest, P.J.; Devantier, Y.; Rutledge, R.G. Mol. Gen. Genomics 270: 78-86.

Year: 2003

Issued by: Laurentian Forestry Centre

Catalog ID: 24499

Language: English

Availability: PDF (request by e-mail)

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PCR amplification with degenerate primers targeted to highly conserved amino acid motifs within the MYB domain was used to demonstrate that black spruce (Picea mariana) possesses a diverse MYB gene family. Amino acid sequence comparisons revealed three broad MYB subfamilies, one of which shares extensive similarity with maize C1, a central regulator of anthocyanin biosynthesis. A cDNA clone encoding a MYBR2R3 protein from P. mariana with high levels of sequence homology to maize C1 was shown to transactivate the Bz2 promoter in combination with maize R in embryonal tissues of both black spruce and larch. Functional dependence on the maize R protein, and the presence of a conserved C-terminal GIDPxTH motif, support the conservation of MYBR2R3 function in conifers, and demonstrate that the basic components of MYBR2R3-dependent transcriptional regulation have been conserved between angiosperms and gymnosperms.