Canadian Forest Service Publications

Comparative activity of three isolates of LdMNPV against two strains of Lymantria dispar. 2004. Ebling, P.M.; Otvos, I.S.; Conder, N. The Canadian Entomologist 136: 737-747.

Year: 2004

Issued by: Pacific Forestry Centre

Catalog ID: 25000

Language: English

Availability: PDF (request by e-mail)

Mark record


Two newly identified geographic isolates of nucleopolyhedrovirus (LdMNPV-H and LdMNPV-J) were evaluated against Disparvirus (LdMNPV-D), a baculovirus insecticide registered in Canada for the control of gypsy moth [Lymantria dispar (L.) (Lepidoptera: Lymantriidae)]. Profiles of HindIII and EcoRI restriction enzyme digests of viral DNA from the three NPV isolates are presented. Viral isolates were bioassayed using an inoculated diet plug method to determine the dose- and time-response of second-instar larvae of both the European and Asian strains of gypsy moth. LdMNPV-D was found to be the most virulent isolate when tested against the European strain of gypsy moth, yielding a LD50 and LD95 of 95 and 774 OBs, respectively. LdMNPV-H was the most virulent isolate when tested against the Asian strain, yielding a LD50 and LD95 of 648 and 8540 OBs, respectively. Time-response data indicates that the three isolates differ very little with respect to their speed of kill (ST50) of either larval strain. These results indicate that both new isolates (LdMNPV-H and LdMNPV-J) should be further investigated for control of the Asian strain of the gypsy moth but not pursued for control of the European strain.