Canadian Forest Service Publications

Sequence and organization of the Neodiprion lecontei nucleopolyhedrovirus genome. 2004. Lauzon, H.A.M.; Lucarotti, C.J.; Krell, P.J.; Feng, Q.; Retnakaran, A.; Arif, B.M. Journal of Virology 78: 7023-7035.

Year: 2004

Issued by: Great Lakes Forestry Centre

Catalog ID: 25007

Language: English

Availability: PDF (request by e-mail)

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The morphological and molecular responses of a midgut-derived cell line of the spruce budworm, Choristoneura fumiferana, to 20-hydroxyecdysone (20E) and the nonsteroidal ecdysone agonist, tebufenozide (RH-5992), were investigated. The cells responded to these compounds by clumping, generating filamentous extensions, increased mortality and expression of the transcription factor, Choristoneura hormone receptor 3 (CHR3). This cell line can be used as a model system to study the mode of action of ecdysone and its agonists. With subsequent passaging in ecdysteroid-containing medium, the degree of clumping increased and the clumping could not be reversed by subculturing in ecdysteroid-free medium. Cell numbers of the adapted cell lines in 20E and RH-5992 containing media were not significantly decreased, compared to the control, but both cell lines accumulated less 14C-labeled RH-5992 and lost the capability of expressing CHR3 in response to these compounds. Taken together, the cell lines appeared to develop a mechanism to adapt to the toxic effects of these compounds.