Canadian Forest Service Publications

Total RNA isolation from Picea mariana dry seed. 2004. Tai, H.; Pelletier, C.; Beardmore, T. Plant Molecular Biology Reporter 22: 93a-93e.

Year: 2004

Issued by: Atlantic Forestry Centre

Catalog ID: 25168

Language: English

CFS Availability: Order paper copy (free)

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Abstract

Isolating RNA from dry conifer seeds can be difficult because of a number of interfering compounds present in seeds. We describe a protocol for total RNA isolation from black spruce dry seeds, which is an adaptation of a method used for mouse myeloma tissues. The extraction relies on selective precipitation of RNA by using lithium chloride.