Canadian Forest Service Publications
Total RNA isolation from Picea mariana dry seed. 2004. Tai, H.; Pelletier, C.; Beardmore, T. Plant Molecular Biology Reporter 22: 93a-93e.
Issued by: Atlantic Forestry Centre
Catalog ID: 25168
Availability: Order paper copy (free)
Isolating RNA from dry conifer seeds can be difficult because of a number of interfering compounds present in seeds. We describe a protocol for total RNA isolation from black spruce dry seeds, which is an adaptation of a method used for mouse myeloma tissues. The extraction relies on selective precipitation of RNA by using lithium chloride.