Canadian Forest Service Publications

Screening of ESTs from Septoria musiva (teleomorph Mycosphaerella populorum) for detection of SSR and PCR-RFLP markers. 2006. Feau, N.; Jacobi, V.; Hamelin, R.C.; Bernier, L. Mol. Ecol. Notes 6: 356-358.

Year: 2006

Available from: Laurentian Forestry Centre

Catalog ID: 26544

Language: English

CFS Availability: PDF (request by e-mail)

Available from the Journal's Web site.
DOI: 10.1111/j.1471-8286.2006.01230.x

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Abstract

The development of an expressed sequence tag (EST) collection for the poplar pathogen Septoria musiva presented an opportunity for the development of locus-specific polymorphic markers. Five microsatellites simple sequence repeat (SSR) and six polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) markers were identified and tested on three S. musiva populations collected in the north-central and northeastern regions of North America. The results obtained support previously published random amplified polymorphic DNA (RAPD) data.

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