Canadian Forest Service Publications

Growth of four serovar of Bacillus thuringiensis (var. Kurstaki, Israelensis, Tenebrionis, and Aizawai) in wastewater sludge. 2007. Yan, S.; Mohammedi, S.; Tyagi, R.D.; Surampalli, R.Y.; Valéro, J.R. Pract. Periodical Hazard. Waste Manag. April 2007: 123-129.

Year: 2007

Issued by: Laurentian Forestry Centre

Catalog ID: 27004

Language: English

Availability: PDF (request by e-mail)

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Abstract

Growth patterns of four different serovar of Bacillus thuringiensis (Bt) (Bt kurstaki, Bt israelensis, Bt tenebrionis, and Bt aizawai) were studied using wastewater sludge as a raw material. A comparative study using synthetic medium (soy) was also performed. It was observed that the four Bt strains gave cell counts of the order of 10⁸ CFU/mL in wastewater sludge and 10⁹ CFU/mL in synthetic medium. The sporulation (% spores) in two media was 90%. The entomotoxicity per unit spore (specific entomotoxicity) was much higher in sludge medium compared to synthhetic medium. Therefore, sludge was a good source of nutrients for growth and toxin production by all types of serovar used in this study.