Canadian Forest Service Publications

Detection and validation of EST-derived SNPs for poplar leaf rust Melampsora medusae f. sp. deltoidae. 2007. Feau, N.; Bergeron, M.J.; Joly, D.L.; Roussel, F. Molecular Ecology Notes 7: 1222-1228.

Year: 2007

Issued by: Laurentian Forestry Centre

Catalog ID: 28061

Language: English

CFS Availability: PDF (request by e-mail)

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Abstract

We report the discovery, characterization and validation of 118 single nucleotide polymorphisms (SNPs) for poplar leaf rust Melampsora medusae f.sp. deltoidae identified using a gene-targeted approach in an expressed sequence tag (EST) library. We developed a genotyping assay using the iPLEXTM primer extension method for two multiplex assays of 28 and 22 SNPs.