Canadian Forest Service Publications

A bilayer plate technique to detect broad-spectrum antagonism in microorganisms and its application to wood-inhabiting fungi. 1973. Etheridge, D.E.; Craig, H.M. Canadian Journal of Microbiology 19(11): 1455-1458.

Year: 1973

Issued by: Pacific Forestry Centre

Catalog ID: 28096

Language: English

Availability: Not available through the CFS (click for more information).

Available from the Journal's Web site.
DOI: 10.1139/m73-236

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A simple method is described for measuring antagonistic activity in microorganisms based on the mycelial growth response of up to 10 isolates of the bioassay fungus in a single agar plate. The technique overcomes disadvantages of existing methods by providing quantitative estimates of interstrain variability in the bioassay fungus as well as the antagonist. Application of the method to a wide range of isolates of Ascocoryne sarcoides (Jacq. ex Gray) Groves and Wilson against three species of decay fungi has permitted the rapid detection of broad-spectrum strains of this fungus which would not have been practical by conventional bioassays