Canadian Forest Service Publications

Cellular response to ecdysone: RNA and protein synthesis in larval tissues of oak silkworm Antheraea pernyi. 1970. Sahota, T.S.; Mansingh, A. Journal of Insect Physiology 16(8): 1649-1654.

Year: 1970

Issued by: Pacific Forestry Centre

Catalog ID: 28256

Language: English

Availability: Not available through the CFS (click for more information).

Available from the Journal's Web site.
DOI: 10.1016/0022-1910(70)90264-7

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The effect of exogenous ecdysone on RNA and protein synthesis in epidermis, fat body, silk gland, and midgut was studied by injecting uridine-3H and leucine-3H into fourth instar larvae of A. pernyi. Autoradiography revealed that RNA synthesis was mostly confined to the nuclei, and that of protein to the cytoplasm of the cells. Ecdysone stimulated the biosynthetic activity in all the four tissues. RNA synthesis increased sharply during the first 90 min, whereas protein synthesis enhanced during 90 to 180 min after ecdysone administration. The stimulatory effect of the hormone was transitory. After reaching their peaks, the rates of biosyntheses fell sharply to near control levels. Though the pattern of stimulation was almost similar in all the tissues, the extent of response of each tissue was different. It is concluded that the primary site of action of ecdysone is the nuclei of cells of several tissues.