Canadian Forest Service Publications

Phylogenetic analysis of Orgyia pseudotsugata single-nucleocapsid nucleopolyhedrovirus. 2007. Jakubowska, A.; van Oers, M.M.; Otvos, I.S.; Vlak, J.M. Virologica Sinica 22(4): 257-265.

Year: 2007

Issued by: Pacific Forestry Centre

Catalog ID: 28291

Language: English

Availability: Not available through the CFS (click for more information).

Available from the Journal's Web site.
DOI: 10.1007/s12250-007-0001-8

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The Douglas-fir tussock moth Orgyia pseudotsugata (Lepidoptera: Lymantriidae) is a frequent defoliator of Douglas-fir and true firs in western USA and Canada. A single nucleopolyhedrovirus (SNPV) isolated from O. pseudotsugata larvae in Canada (OpSNPV) was previously analyzed via its polyhedrin gene, but is phylogenetic status was ambiguous. Sequences of four conserved baculovirus genes, polyhedrin, lef-8, pif-2 and dpol, were amplified from OpSNPV DNA in polymerase chain reactions using degenerate primer sets and their sequences were analyzed phylogenetically. The analysis revealed that OpSNPV belongs to group II NPVs and is most closely related to SNPVs that infect O. ericae and O. anartoides, respectively. These results show the need for multiple, concatenated gene phylogenies to classify baculoviruses.