Canadian Forest Service Publications
Yolk deposition in Douglas-fir beetle oöcytes: Possible role of RNA synthesis in the follicular epithelium. 1973. Sahota, T.S. Journal of Insect Physiology 19(5): 1087-1095.
Issued by: Pacific Forestry Centre
Catalog ID: 28707
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RNA synthesis and morphological changes in the follicular epithelial cells of oöcytes of Douglas-fir beetle, Dendroctonus pseudotsugae were studied during the reproductive phase. Inhibition of synthesis of DNA dependent RNA by actinomycin D injections blocked yolk deposition in the oöcytes as well as oviposition within the normal period. A mixture of radioactively labelled haemolymph and ovarial proteins was deposited as yolk proteins in the oöcytes of normal beetles. Such proteins were not deposited in the oöcytes of females injected with actinomycin D; the blockage of yolk deposition persisted even when such females were treated extraneously with juvenile hormone.