Canadian Forest Service Publications

Genomic organization of Ilellcoverpa armigera singlc-nucleocap sid nucleopolyhcd rovirus. 2000. Chen, X.; Li, M.; Sun, X.; Arif, B.M.; Hu, Z.; Vlak, J.M. Archives of Virology 145: 2539 - 2555.

Year: 2000

Available from: Great Lakes Forestry Centre

Catalog ID: 28921

Language: English

CFS Availability: PDF (request by e-mail)

Abstract

The genome organization ofthe Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV) was investigated and compared to that of other baculoviruses.A detailed physical map was constructed for the restriction enzymes BamHI, BgfI l, EcoRr, Hindlll . Kpnl, PstI, Sad and XlwI. The 130.1kilobase pairs (kb) long viral genome was cloned as restriction fragments into a plasmid library. which covered 97.5% of the viral genome. From this plasmid library about 45 kb of dispersed sequence information was generated. Fiftythree putative open reading frames (ORFs) with homology to ORFs of other baculoviruses were identified and their location on the genome of HearNPV was determined. The arrangement of the ORFs along the HearNPV genome is significantly different from the multiple-nucleocapsid NPVs. AcMNPV, BmNPV and OpMNPV, which have a highly collinear genome, and the granulovirus XcGY. In contrast the genomes of HearNPV and SeMNPV are highly collinear. This close relatedness between anMNPV and an SNPV is supported by the phylogeny of specific genes of these two viruses and suggests that the morphotype (S or M) is only a taxonomic and not a phylogenetic denominator. The data presented here also give further support to the view that the gene distribution along baculovirus gcnomcs can be used as a character to describe baculo virus relatedness independent of gene phylogeny. Five homologous regions (hrs) were located on the genome of HearNPY. This is also the first report of the existence of !Irs in SNPVs and this observation suggests that hrs are a common feature ofbaculovirus genomes.

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