Canadian Forest Service Publications

Immunochemical characterization of the entomopathogenic fungus Beauveria bassiana. 1991. Tan, Y.; Ekramoddoullah, A.K.M. Journal of Invertebrate Pathology 57: 269-276.

Year: 1991

Issued by: Pacific Forestry Centre

Catalog ID: 3061

Language: English

Availability: PDF (request by e-mail)

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Abstract

Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of crude extracts from the mycelium of five Beauveria bassiana isolates contained several proteins which were used to produce antibodies in BALB/c mice. The antibody response to these proteins was good and enzyme-linked immunoassay showed a wide antigenic cross-reactivity among the isolates. An isolate from Canada and one from the United States were the most cross-reactive while two isolates from China were the least cross-reactive. Both SDS-PAGE and Western immunoblot showed that each of the five isolates had a unique protein and antigenic profile. The immunoblot analyses also revealed the presence of a unique antigen in one of the Chinese isolates.