Canadian Forest Service Publications

Isozyme analysis of Chondrostereum purpureum used as a mycoherbicide. 1991. Shamoun, S.F.; Ekramoddoullah, A.K.M.; Wall, R.E. Canadian Journal of Plant Pathology 13(3): 285.

Year: 1991

Available from: Pacific Forestry Centre

Catalog ID: 3119

Language: English

CFS Availability: Order paper copy (free)

Abstract

Isozyme analysis was used to examine the variation among 17 Chondrostereum purpureum (Pers.:Fr. Pouzar) isolates used as a potential forest mycoherbicide. Native proteins from mycelial extracts of the isolates from different host plants across Canada were electrophoretically tested for the activity of 13 different isozyme systems on polyacrylamide gel. Eight of the systems produced positive activities: acid phosphatase (E.C.3.1.3.2), alkaline phosphatase (E.C.3.1.3.1), ß-esterase (E.C.3.1.1.1), ß-glucosidase (E.C.3.2.1.21), malate dehydrogenase (E.C.1.1.1.37), peroxidase (E.C.1.11.1.7), phosphoglucomutase (E.C.2.7.5.1), and polyphenoloxidase (E.C.1.14.18.1). All eight enzyme systems were further submitted to polyacrylamide gel electrophoresis using standardized protein samples of 150 micrograms. Although the relative migrations of bands were generally similar among isolates for a given enzyme system, suggesting the existence of monomorphic loci, they varied noticeably in their intensities. A laser scanner interfaced with appropriate software was used to quantify band intensities and thereby characterize individual C. purpureum isolates. These biochemical markers may be related to virulence on the basis of ongoing pathogenicity studies.

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