Canadian Forest Service Publications

Isozyme patterns of Chondrostereum purpureum by vertical polyacrylamide gel electrophoresis (Abstract) 1991. Shamoun, S.F.; Thom, D.C.; Wall, R.E.; Ekramoddoullah, A.K.M. Page 99 in A.S. McClay, Editor. Proceedings of the workshop on biological control of pests in Canada, October 11-12, 1990, Calgary, Alberta. Alberta Environmental Centre, Vegreville, AB.

Year: 1991

Available from: Pacific Forestry Centre

Catalog ID: 3120

Language: English

CFS Availability: Order paper copy (free)

Abstract

The biochemical characterization of 17 isolates of Chondrostereum purpureum (Fr.) Pouzar, a potential forest mycoherbicide, was attempted by detection of isozymes using polyacrylamide gel electrophoresis (P.A.G.E.). Isolates were collected from different plant hosts across Canada and grown for two weeks under controlled conditions. The mycelium for each isolate was collected, freeze dried and homogenized in cold acetone. For this preparation, a protein extract was derived and its protein concentration determined by the Bradford standard protein assay. The banding patterns of general proteins and 13 enzyme systems were visually observed by vertical P.A.G.E. The eight enzyme systems which produced positive activities were once again subject to P.A.G.E., but using C. purpureum protein samples standardized to 150 mg protein/well). Although the relative migrations of bands are generally similar between isolates for a given enzyme system, they varied noticeably in their intensities (especially acid phosphatase, beta-esterase, peroxidase, beta-glycosidase and polyphenoloxidase). Results suggest the possibility of characterizing activity patterns for each isolate but must first be correlated with pathogenicity studies before the virulence of a given strain can be quantified using biochemical assays.

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