Canadian Forest Service Publications
Genetic variability among isolates of Chondrostereum purpureum. 1991. Shamoun, S.F.; Vrain, T.C.; Wall, R.E. Canadian Journal of Plant Pathology 13(3): 285.
Issued by: Pacific Forestry Centre
Catalog ID: 3121
Available from the Journal's Web site. †
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Chondrostereum purpureum is an important pathogen of orchard crops and is also under evaluation as a mycoherbicide for unwanted brush species in forestry. There is a need to understand the genetic variability of the fungus in order to place appropriate limits on transfer of isolates among regions or continents. If the fungus is used as a mycoherbicide, it will also be necessary to characterize specific strains, through genetic fingerprinting, and distinguish them from wild populations. Recently, 10 isolates from Canada, Europe and New Zealand were examined. Nucleic acid was extracted from freeze-dried mycelium by standard phenol chloroform technique, and precipitated in ethanol. The Internal Transcribed Spacer (ITS) of the ribosomal repeat was amplified using universal primers. The amplified product was digested with seven endonuclease restriction enzymes. There were common restriction sites among all isolates except the New Zealand isolate, indicating little genetic variability in the species.