Canadian Forest Service Publications

Isolation of inhibitor-free poly(A+) RNA from Pinus monticola and Pinus lambertiana seedlings. 1992. Baker, S.M.; White, E.E.; Misra, S. Canadian Journal of Forest Research 22: 622-624.

Year: 1992

Issued by: Pacific Forestry Centre

Catalog ID: 3136

Language: English

Availability: Order paper copy (free)

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Abstract

Inhibition of in vitro translation by material(s) that co-purifies with RNA obtained from 6- to 12-week-old western white pine (Pinus monticola Dougl. ex Lamb.) and sugar pine (Pinus lambertiana Dougl.) seedlings was demonstrated. A method that consistently produces inhibitor-free poly(A+) RNA from small amounts of total RNA isolated from these seedlings is described.