Canadian Forest Service Publications
Identification of the Phytoplasma Associated with Wheat Blue Dwarf Disease in China. 2010. Wu, Y.; Hao, X.; Li, Z.; Gu, P; An, F.; Xiang, J; Wang, H; Luo, Z.; Liu, J.-J.; Xiang, Y. Plant Disease 94(8): 977-985.
Available from: Pacific Forestry Centre
Catalog ID: 31769
CFS Availability: PDF (request by e-mail)
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Wheat blue dwarf disease (WBD) was first reported in China in the 1960s. It has caused severe losses on several occasions in winter wheat (Triticum aestivum) in northwestern China, and the nature of the pathogenic agent has been unknown. Here we have shown that WBD was caused by a 16SrI-C phytoplasma transmitted by Psammotettix striatus. This finding was based on molecular diagnostics, insect transmission trials, and host-range determination. Portions of the 16S rRNA and ribosomal protein (rp) genes, rpsS (rps19), rplV (rpl22), and rpsC (rps3), were amplified from DNA samples of WBD-infected wheat seedlings by polymerase chain reaction (PCR) utilizing phytoplasma specific primer pairs. The nucleotide sequences of these amplicons showed high identity to these genes from phytoplasma strains in the aster yellows group (16SrI). Pairwise nucleotide sequence identities of WBD 16S rDNA compared to representative genes of 16SrI group strains ranged from 98.9 to 99.9%, whereas compared to 17 other phytoplasma groups (16SrII to 16SrXVIII), sequence identity ranged from 88.6 to 96.0%. Similarly, the sequence identities of rps19, rpl22, and rps3 between WBD and 16SrI group strains varied from 96.6 to 99.7%, but only 60.3 to 65% between WBD and other phytoplasma groups. Phylogenetic analyses were carried out on sequences from 16S rRNA and ribosomal protein genes (rps19, rpl22, and rps3), respectively, and both results indicated that WBD phytoplasma was a member of the 16SrI group and most closely related to subgroup 16SrI-C. WBD-infected P. striatus were present in wheat fields with WBD, and phytoplasma infection was verified by PCR detection followed by DNA sequencing. Insect transmission trials confirmed that P. striatus transmitted the WBD phytoplasmal agent from infected wheat to healthy wheat seedlings and seven other different plant species in the greenhouse. A survey of various weed species near WBD-infected wheat fields found 10 plant species in seven families to be positive for the presence of WBD phytoplasma.
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