Canadian Forest Service Publications

Analytical study of azadirachtin and 3-Tigloylazadirachtol residues in foliage and phloem of hardwood tree species by liquid chromatography-electrospray mass spectrometry. 2011. Grimalt, S.; Thompson, D. G.; Coppens, M.; Chartrand, D.T.; Shorney, T.; Meating, J.; Scarr, T. Journal of Agricultural and Food Chemistry 59:8070-8077.

Year: 2011

Issued by: Great Lakes Forestry Centre

Catalog ID: 32669

Language: English

Availability: PDF (request by e-mail)

Available from the Journal's Web site.
DOI: 10.1021/jf2023947

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A rapid and sensitive LC-ESI-MS method has been developed and validated for the quantitation of azadirachtin and 3-tigloylazadirachtol in deciduous tree matrices. The method involves automated extraction and simultaneous cleanup using an accelerated solvent technique with the matrix dispersed in solid phase overalayer ofprimary—secondary amine silica. The limits of quantification were 0.02 mg/kg for all matrices with the exception ofNorway maple foliage (0.05 mg/kg). Validation at three levels (0.02, 0.1, and 1mg/kg), demonstrated satisfactory recoveries (71—103%) with relative standard deviation <20%. Two in-source fragment ions were used for confirmation at levels above 0.1 mg/kg. Over a period of several months, quality control analyses showed the technique to be robust and effective in tracking the fate of these natural botanical insecticides following systemic injection intovarious treespecies for control of invasive insect pest species such as the emerald ash borer and Asian longhorned beetle.