Canadian Forest Service Publications

The influence of nitrogenous compounds on growth of pythium species. 1967. Agnihotri, V.P.; Vaartaja,O. Canadian Journal of Microbiology 13:1509-1519.

Year: 1967

Issued by: Great Lakes Forestry Centre

Catalog ID: 33116

Language: English

Availability: PDF (request by e-mail)

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The utilization of N compounds by P. ultimum Trow (strain I and II). P. rostratum Butler, and P. irregulare Huisman was examined in a chemically defined medium under controlled conditions in surface culture. All species were able to metabolize nitrate, ammonium, and organic nitrogen. and the amount of growth varied with the nitrogen source. In general, yeast extract, peptone. glycine, serine, histidine, cysteine, asparagine, aspartic acid, and glutamic acid supported favorable growth, whereas -y-aminobutyric acid, threonine, and alanine supported poor growth of these fungi. The addition of succinic acid at 0.02 M concentration to ammonium compounds further increased growth of four isolates. Preferential utilization of amino acids from a given mixture was recorded using paper chromatographic techniques. All four isolates gave more vegetative growth on mixtures of amino acids than when they were supplied singly.